1JF5

CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A

Summary for 1JF5

• Entry DOI: 10.2210/pdb1jf5/pdb
• Related: 1BVZ 1JF6
• Descriptor: ALPHA AMYLASE II, CALCIUM ION (2 entities in total)
• Functional Keywords: beta/alpha barrel, hydrolase
• Biological source: Thermoactinomyces vulgaris
• Total number of polymer chains: 2
• Total formula weight: 135036.19

Authors

Ohtaki, A.,Kondo, S.,Shimura, Y.,Tonozuka, T.,Sakano, Y.,Kamitori, S. (deposition date: 2001-06-20, release date: 2002-05-22, Last modification date: 2021-11-10)

• Primary citation: Ohtaki, A.,Kondo, S.,Shimura, Y.,Tonozuka, T.,Sakano, Y.,Kamitori, S.; Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structures of the mutant TVAIIs, F286A and F286Y, and kinetic analyses of the Phe286-replaced mutant TVAIIs; CARBOHYDR.RES., 334:309-313, 2001; PubMed: 11527532; DOI: 10.1016/S0008-6215(01)00190-2

Experimental method

X-RAY DIFFRACTION (3.2 Å)