1JF5
CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.600, 118.050, 113.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.390 - 3.200 |
R-factor | 0.213 * |
Rwork | 0.214 |
R-free | 0.27500 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 24.900 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 * |
High resolution limit [Å] | 3.200 |
Rmerge | 0.122 |
Total number of observations | 130745 * |
Number of reflections | 25420 |
<I/σ(I)> | 6.2 |
Completeness [%] | 99.8 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 11 * | Kamitori, S., (1999) J.Mol.Biol., 287, 907. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 2 (%(w/v)) | |
2 | 1 | reservoir | 2.5 (mM) | ||
3 | 1 | reservoir | MES | 20 (mM) | |
4 | 1 | drop | protein | 20 (mg/ml) |