1J6Z

UNCOMPLEXED ACTIN

1J6Z の概要

• エントリーDOI: 10.2210/pdb1j6z/pdb
• 関連するPDBエントリー: 1ATN 1DB0 1DGA 1EQY 2BTF
• 分子名称: ACTIN ALPHA 1, CALCIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: actin, tetramethylrhodamine-5-maleimide, adp-state, contractile protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• 細胞内の位置: Cytoplasm, cytoskeleton: P68135
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43026.82

構造登録者

Otterbein, L.R.,Graceffa, P.,Dominguez, R. (登録日: 2001-05-15, 公開日: 2001-08-15, 最終更新日: 2023-08-16)

主引用文献

Otterbein, L.R.,Graceffa, P.,Dominguez, R.
The crystal structure of uncomplexed actin in the ADP state.
Science, 293:708-711, 2001

PubMed Abstract: The dynamics and polarity of actin filaments are controlled by a conformational change coupled to the hydrolysis of adenosine 5'-triphosphate (ATP) by a mechanism that remains to be elucidated. Actin modified to block polymerization was crystallized in the adenosine 5'-diphosphate (ADP) state, and the structure was solved to 1.54 angstrom resolution. Compared with previous ATP-actin structures from complexes with deoxyribonuclease I, profilin, and gelsolin, monomeric ADP-actin is characterized by a marked conformational change in subdomain 2. The successful crystallization of monomeric actin opens the way to future structure determinations of actin complexes with actin-binding proteins such as myosin.

PubMed: 11474115
DOI: 10.1126/science.1059700

実験手法

X-RAY DIFFRACTION (1.54 Å)