1J4V

CYANOVIRIN-N

Summary for 1J4V

• Entry DOI: 10.2210/pdb1j4v/pdb
• Related: 2EZM 2EZN 3EZM
• Descriptor: CYANOVIRIN-N (1 entity in total)
• Functional Keywords: cyanovirin-n, hiv-inactivating, domain-swapped dimer, dipolar couplings / conjoined rigid body-torsion angle dynamics, immune system
• Biological source: Nostoc ellipsosporum
• Total number of polymer chains: 2
• Total formula weight: 22044.18

Authors

Clore, G.M.,Bewley, C.A. (deposition date: 2001-11-21, release date: 2002-03-06, Last modification date: 2024-11-06)

Primary citation

Clore, G.M.,Bewley, C.A.
Using conjoined rigid body/torsion angle simulated annealing to determine the relative orientation of covalently linked protein domains from dipolar couplings.
J.Magn.Reson., 154:329-335, 2002

PubMed Abstract: A simple and robust method for determining the relative orientations of covalently linked protein domains using conjoined rigid body/torsion angle dynamics simulated annealing on the basis of residual dipolar couplings is presented. In this approach each domain is treated as a rigid body and the relevant degrees of conformational freedom are restricted to the backbone torsion angles (phi, psi) of the linker between the domains. By this means translational information afforded by the presence of an intact linker is preserved. We illustrate this approach using the domain-swapped dimer of the HIV-inactivating protein cyanovirin-N as an example.

PubMed: 11846592
DOI: 10.1006/jmre.2001.2489

Experimental method

SOLUTION NMR