Crystal Structure of the E.coli SeqA protein complexed with N6-methyladenine- guanine mismatch DNA

Summary for 1J3E

Descriptor5'-D(*AP*AP*GP*GP*(6MA)P*TP*CP*CP*AP*A)-3', 5'-D(*TP*TP*GP*GP*AP*GP*CP*CP*TP*T)-3', SeqA protein, ... (4 entities in total)
Functional Keywordsprotein-dna complex, recognition of hemimethylated dna, mismatched dna, replication, riken structural genomics/proteomics initiative, rsgi, structural genomics, replication-dna complex, replication/dna
Biological sourceEscherichia coli
Total number of polymer chains3
Total molecular weight19105.95
Fujikawa, N.,Kurumizaka, H.,Nureki, O.,Tanaka, Y.,Yamazoe, M.,Hiraga, S.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2003-01-24, release date: 2004-05-18, Last modification date: 2011-07-13)
Primary citation
Fujikawa, N.,Kurumizaka, H.,Nureki, O.,Tanaka, Y.,Yamazoe, M.,Hiraga, S.,Yokoyama, S.
Structural and biochemical analyses of hemimethylated DNA binding by the SeqA protein
Nucleic Acids Res., 32:82-92, 2004
PubMed: 14704346 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkh173
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.301271.8%6.1%1.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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