1J3E
Crystal Structure of the E.coli SeqA protein complexed with N6-methyladenine- guanine mismatch DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9740 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.432, 65.061, 77.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.2302 |
| Rwork | 0.230 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 6126 | |
| Completeness [%] | 97.3 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Ca-acetate, Na-cacodylate, PEG8K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | Ca-acetate | ||
| 3 | 1 | 1 | Na-cacodylate | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | Ca-acetate | ||
| 6 | 1 | 2 | Na-cacodylate |
