1IS7

Crystal structure of rat GTPCHI/GFRP stimulatory complex

1IS7 の概要

• エントリーDOI: 10.2210/pdb1is7/pdb
• 関連するPDBエントリー: 1IS8
• 分子名称: GTP Cyclohydrolase I, GTP Cyclohydrolase I Feedback Regulatory Protein, POTASSIUM ION, ... (5 entities in total)
• 機能のキーワード: enzyme-regulatory protein complex, hydrolase-protein binding complex, hydrolase/protein binding
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasm (By similarity): P22288; Nucleus (By similarity): P70552
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 357071.84

構造登録者

Maita, N.,Okada, K.,Hatakeyama, K.,Hakoshima, T. (登録日: 2001-11-18, 公開日: 2002-02-20, 最終更新日: 2023-12-27)

主引用文献

Maita, N.,Okada, K.,Hatakeyama, K.,Hakoshima, T.
Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP.
Proc.Natl.Acad.Sci.USA, 99:1212-1217, 2002

PubMed Abstract: In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers. Each GFRP pentamer forms a symmetrical five-membered ring similar to beta-propeller. Five phenylalanine molecules are buried inside each interface between GFRP and GTPCHI, thus enhancing the binding of these proteins. The complex structure suggests that phenylalanine-induced GTPCHI x GFRP complex formation enhances GTPCHI activity by locking the enzyme in the active state.

PubMed: 11818540
DOI: 10.1073/pnas.022646999

実験手法

X-RAY DIFFRACTION (2.8 Å)