1IS7
Crystal structure of rat GTPCHI/GFRP stimulatory complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 124.151, 111.723, 126.108 |
Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.228 * |
Rwork | 0.228 |
R-free | 0.26400 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 23.200 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.940 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.112 * | 0.578 * |
Total number of observations | 589690 * | |
Number of reflections | 83068 | |
<I/σ(I)> | 5.5 | 1.2 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 283 | Maita, N., (2001) Acta Crystallogr., D57, 1153. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | MPD | 24 (%(v/v)) | |
3 | 1 | reservoir | Tris-HCl | 75 (mM) | pH7.5 |
4 | 1 | reservoir | 50 (mM) | ||
5 | 1 | reservoir | phenylalanine | 5 (mM) |