1IO4

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

1IO4 の概要

• エントリーDOI: 10.2210/pdb1io4/pdb
• 関連するPDBエントリー: 1CL3 1CMO 1CO1 1E50 1HJB 1HJC 2JHB
• 分子名称: CSF-1R PROMOTER, CAAT/ENHANCER BINDING PROTEIN BETA, RUNT-RELATED TRANSCRIPTION FACTOR 1, ... (7 entities in total)
• 機能のキーワード: protein-dna complex, transcription factor, bzip, runx, runt, c/ebp, cbf, core binding factor, aml1, aml, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P17676 Q03347; Nucleus (Potential): Q08024
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 65658.31

構造登録者

Tahirov, T.H.,Ogata, K. (登録日: 2001-01-10, 公開日: 2001-03-12, 最終更新日: 2023-12-27)

主引用文献

Tahirov, T.H.,Inoue-Bungo, T.,Morii, H.,Fujikawa, A.,Sasaki, M.,Kimura, K.,Shiina, M.,Sato, K.,Kumasaka, T.,Yamamoto, M.,Ishii, S.,Ogata, K.
Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta.
Cell(Cambridge,Mass.), 104:755-767, 2001

PubMed Abstract: The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta subunits are essential for differentiation of hematopoietic and bone cells, and their mutation is intimately related to the development of acute leukemias and cleidocranial dysplasia. Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(bZip)-DNA, and AML1/Runx-1/CBFalpha(Runt domain)-DNA complexes. The hydrogen bonding network formed among CBFalpha(Runt domain) and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the allosteric regulation mechanism of CBFalpha(Runt domain)-DNA binding by CBFbeta. The point mutations of CBFalpha related to the aforementioned diseases were also mapped and their effect on DNA binding is discussed.

PubMed: 11257229
DOI: 10.1016/S0092-8674(01)00271-9

実験手法

X-RAY DIFFRACTION (3 Å)