1IIK

CRYSTAL STRUCTURE OF THE TRANSTHYRETIN MUTANT TTR Y114C-DATA COLLECTED AT CRYO TEMPERATURE

Summary for 1IIK

• Entry DOI: 10.2210/pdb1iik/pdb
• Related: 1III 1IIM 1IIN
• Descriptor: TRANSTHYRETIN, BETA-MERCAPTOETHANOL (3 entities in total)
• Functional Keywords: greek key, beta barrel, transport protein
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P02766
• Total number of polymer chains: 2
• Total formula weight: 27903.46

Authors

Eneqvist, T.,Olofsson, A.,Ando, Y.,Lundgren, E.,Sauer-Eriksson, A.E. (deposition date: 2001-04-23, release date: 2002-11-15, Last modification date: 2023-08-16)

Primary citation

Eneqvist, T.,Olofsson, A.,Ando, Y.,Miyakawa, T.,Katsuragi, S.,Jass, J.,Lundgren, E.,Sauer-Eriksson, A.E.
Disulfide-Bond Formation in the Transthyretin Mutant Y114C Prevents Amyloid Fibril Formation in Vivo and in Vitro
Biochemistry, 41:13143-13151, 2002

PubMed Abstract: The Y114C mutation in human transthyretin (TTR) is associated with a particular form of familial amyloidotic polyneuropathy. We show that vitreous aggregates ex vivo consist of either regular amyloid fibrils or disordered disulfide-linked precipitates that maintain the ability to bind Congo red. Furthermore, we demonstrate in vitro that the ATTR Y114C mutant exists in three forms: one unstable but nativelike tetrameric form, one highly aggregated form in which a network of disulfide bonds is formed, and one fibrillar form. The disulfide-linked aggregates and the fibrillar form of the mutant can be induced by heat induction under nonreduced and reduced conditions, respectively. Both forms are recognized by the amyloid specific antibody MAB(39-44). In a previous study, we have linked exposure of this epitope in TTR to a three-residue shift in beta-strand D. The X-ray crystallographic structure of reduced tetrameric ATTR Y114C shows a structure similar to that of the wild type but with a more buried position of Cys10 and with beta-mercaptoethanol associated with Cys114, verifying the strong tendency for this residue to form disulfide bonds. Combined with the ex vivo data, our in vitro findings suggest that ATTR Y114C can lead to disease either by forming regular unbranched amyloid fibrils or by forming disulfide-linked aggregates that maintain amyloid-like properties but are unable to form regular amyloid fibrils.

PubMed: 12403615
DOI: 10.1021/bi025800w

Experimental method

X-RAY DIFFRACTION (2 Å)