1IG7

Msx-1 Homeodomain/DNA Complex Structure

Summary for 1IG7

• Entry DOI: 10.2210/pdb1ig7/pdb
• Descriptor: 5'-D(*CP*AP*CP*TP*AP*AP*TP*TP*GP*AP*AP*GP*G)-3', 5'-D(P*TP*CP*CP*TP*TP*CP*AP*AP*TP*TP*AP*GP*TP*GP*AP*C)-3', Homeotic protein Msx-1, ... (4 entities in total)
• Functional Keywords: helix-turn-helix, transcription-dna complex, transcription/dna
• Biological source: Mus musculus (house mouse)
• Cellular location: Nucleus: P13297
• Total number of polymer chains: 3
• Total formula weight: 15786.88

Authors

Hovde, S.,Abate-Shen, C.,Geiger, J.H. (deposition date: 2001-04-17, release date: 2001-04-23, Last modification date: 2024-04-03)

Primary citation

Hovde, S.,Abate-Shen, C.,Geiger, J.H.
Crystal structure of the Msx-1 homeodomain/DNA complex
Biochemistry, 40:12013-12021, 2001

PubMed Abstract: The Msx-1 homeodomain protein plays a crucial role in craniofacial, limb, and nervous system development. Homeodomain DNA-binding domains are comprised of 60 amino acids that show a high degree of evolutionary conservation. We have determined the structure of the Msx-1 homeodomain complexed to DNA at 2.2 A resolution. The structure has an unusually well-ordered N-terminal arm with a unique trajectory across the minor groove of the DNA. DNA specificity conferred by bases flanking the core TAAT sequence is explained by well ordered water-mediated interactions at Q50. Most interactions seen at the TAAT sequence are typical of the interactions seen in other homeodomain structures. Comparison of the Msx-1-HD structure to all other high resolution HD-DNA complex structures indicate a remarkably well-conserved sphere of hydration between the DNA and protein in these complexes.

PubMed: 11580277
DOI: 10.1021/bi0108148

Experimental method

X-RAY DIFFRACTION (2.2 Å)