1IG7

Msx-1 Homeodomain/DNA Complex Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	1997-05-20
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	33.600, 60.960, 82.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.200
R-factor	0.205
Rwork	0.205
R-free	0.26800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Even-Skipped Homeodomain/DNA complex
RMSD bond length	0.019 *
RMSD bond angle	2.174 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.150 *	2.000
Rmerge	0.062	0.298 *
Number of reflections	11219 *
<I/σ(I)>	17.3
Completeness [%]	98.9	99.8 *
Redundancy	11	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	PEG 4000, Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	1	Sodium Acetate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	12 (%)
2	1	reservoir	sodium acetate	0.1 (M)