1IF1

INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA

Summary for 1IF1

• Entry DOI: 10.2210/pdb1if1/pdb
• Descriptor: DNA (26-MER), PROTEIN (INTERFERON REGULATORY FACTOR 1) (3 entities in total)
• Functional Keywords: complex (dna-binding protein-dna), transcription factor, interferon regulation, helix-turn-helix, transcription-dna complex, transcription/dna
• Biological source: Mus musculus (house mouse); More
• Cellular location: Nucleus : P15314
• Total number of polymer chains: 4
• Total formula weight: 42811.56

Authors

Escalante, C.R.,Yie, J.,Thanos, D.,Aggarwal, A. (deposition date: 1997-09-12, release date: 1998-02-25, Last modification date: 2024-02-07)

Primary citation

Escalante, C.R.,Yie, J.,Thanos, D.,Aggarwal, A.K.
Structure of IRF-1 with bound DNA reveals determinants of interferon regulation.
Nature, 391:103-106, 1998

PubMed Abstract: The family of interferon regulatory factor (IRF) transcription factors is important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. The IRF family is characterized by a unique 'tryptophan cluster' DNA-binding region. Here we report the crystal structure of the IRF-1 region bound to the natural positive regulatory domain I (PRD I) DNA element from the interferon-beta promoter. The structure provides the first three-dimensional view of a member of the growing IRF family, revealing a new helix-turn-helix motif that latches onto DNA through three of the five conserved tryptophans. The motif selects a short GAAA core sequence through an obliquely angled recognition helix, with an accompanying bending of the DNA axis in the direction of the protein. Together, these features suggest a basis for the occurrence of GAAA repeats within IRF response elements and provide clues to the assembly of the higher-order interferon-beta enhancesome.

PubMed: 9422515
DOI: 10.1038/34224

Experimental method

X-RAY DIFFRACTION (3 Å)