1HPT

THREE-DIMENSIONAL STRUCTURE OF A RECOMBINANT VARIANT OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL TYPE)

1HPT の概要

• エントリーDOI: 10.2210/pdb1hpt/pdb
• 分子名称: PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL TYPE) VARIANT 3 (2 entities in total)
• 機能のキーワード: serine protease inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P00995
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6347.09

構造登録者

Hecht, H.J.,Szardenings, M.,Collins, J.,Schomburg, D. (登録日: 1992-03-27, 公開日: 1993-10-31, 最終更新日: 2024-10-16)

主引用文献

Hecht, H.J.,Szardenings, M.,Collins, J.,Schomburg, D.
Three-dimensional structure of a recombinant variant of human pancreatic secretory trypsin inhibitor (Kazal type).
J.Mol.Biol., 225:1095-1103, 1992

PubMed Abstract: A modified version of the human pancreatic trypsin inhibitor (PSTI), generated in a protein-design project, has been crystallized in spacegroup P4(3) with lattice constants a = 40.15 A, c = 33.91 A. The structure has been solved by molecular replacement. Refinement of the structure by simulated annealing and conventional restrained least-squares yielded for 8.0 to 2.3 A data a final R-value of 19.1%. Differences to the known structures of porcine PSTI complexed with trypsinogen and modified human PSTI complexed with chymotrypsinogen occur at the flexible N-terminal part of the molecule. These differences are influenced by crystal packing, as are low temperature factors for the binding loop. The geometry of the binding loop is similar to the complexed structures.

PubMed: 1613792
DOI: 10.1016/0022-2836(92)90107-U

実験手法

X-RAY DIFFRACTION (2.3 Å)