1HCQ

THE CRYSTAL STRUCTURE OF THE ESTROGEN RECEPTOR DNA-BINDING DOMAIN BOUND TO DNA: HOW RECEPTORS DISCRIMINATE BETWEEN THEIR RESPONSE ELEMENTS

1HCQ の概要

• エントリーDOI: 10.2210/pdb1hcq/pdb
• 分子名称: DNA (5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*CP*AP*GP*TP*GP*AP*CP*CP*T P*G)-3'), DNA (5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*CP*TP*GP*TP*GP*AP*CP*CP*T P*G)-3'), PROTEIN (ESTROGEN RECEPTOR), ... (5 entities in total)
• 機能のキーワード: protein-dna complex, complexed with drug, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Nucleus. Isoform 3: Nucleus: P03372
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 61356.59

構造登録者

Schwabe, J.W.R.,Chapman, L.,Finch, J.T.,Rhodes, D. (登録日: 1995-01-04, 公開日: 1995-11-23, 最終更新日: 2024-11-06)

主引用文献

Schwabe, J.W.,Chapman, L.,Finch, J.T.,Rhodes, D.
The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements.
Cell(Cambridge,Mass.), 75:567-578, 1993

PubMed Abstract: The nuclear hormone receptors are a superfamily of ligand-activated DNA-binding transcription factors. We have determined the crystal structure (at 2.4 A) of the fully specific complex between the DNA-binding domain from the estrogen receptor and DNA. The protein binds as a symmetrical dimer to its palindromic binding site consisting of two 6 bp consensus half sites with three intervening base pairs. This structure reveals how the protein recognizes its own half site sequence rather than that of the related glucocorticoid receptor, which differs by only two base pairs. Since all nuclear hormone receptors recognize one or the other of these two consensus half site sequences, this recognition mechanism applies generally to the whole receptor family.

PubMed: 8221895
DOI: 10.1016/0092-8674(93)90390-C

実験手法

X-RAY DIFFRACTION (2.4 Å)