1HBZ

Catalase from Micrococcus lysodeikticu

Summary for 1HBZ

• Entry DOI: 10.2210/pdb1hbz/pdb
• Descriptor: CATALASE, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (4 entities in total)
• Functional Keywords: oxidoreductase, peroxidase, iron, heme hydrogen peroxide
• Biological source: MICROCOCCUS LYSODEIKTICUS
• Total number of polymer chains: 1
• Total formula weight: 57019.43

Authors

Murshudov, G.N.,Grebenko, A.I.,Barynin, V.V.,Braningen, J.,Wilson, K.S.,Dauter, Z.,Melik-Adamyan, W.R. (deposition date: 2001-04-24, release date: 2001-05-24, Last modification date: 2024-05-08)

Primary citation

Murshudov, G.N.,Melik-Adamyan, W.R.,Grebenko, A.I.,Barynin, V.V.,Vagin, A.A.,Vainshtein, B.K.,Dauter, Z.,Wilson, K.S.
Three-Dimensional Structure of Catalase from Micrococcus Lysodeikticus at 1.5A Resolution
FEBS Lett., 312:127-, 1992

PubMed Abstract: The three-dimensional crystal structure of catalase from Micrococcus lysodeikticus has been solved by multiple isomorphous replacement and refined at 1.5 A resolution. The subunit of the tetrameric molecule of 222 symmetry consists of a single polypeptide chain of about 500 amino acid residues and one haem group. The crystals belong to space group P4(2)2(1)2 with unit cell parameters a = b = 106.7 A, c = 106.3 A, and there is one subunit of the tetramer per asymmetric unit. The amino acid sequence has been tentatively determined by computer graphics model building and comparison with the known three-dimensional structure of beef liver catalase and sequences of several other catalases. The atomic model has been refined by Hendrickson and Konnert's least-squares minimisation against 94,315 reflections between 8 A and 1.5 A. The final model consists of 3,977 non-hydrogen atoms of the protein and haem group, 426 water molecules and one sulphate ion. The secondary and tertiary structures of the bacterial catalase have been analyzed and a comparison with the structure of beef liver catalase has been made.

PubMed: 1426241
DOI: 10.1016/0014-5793(92)80919-8

Experimental method

X-RAY DIFFRACTION (1.5 Å)