1HBZ
Catalase from Micrococcus lysodeikticu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 287 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 106.700, 106.700, 106.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.500 |
Rwork | 0.092 |
R-free | 0.12000 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 0.001 * |
Data scaling software | MOSFLM |
Refinement software | REFMAC (5.0.36) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 1.540 |
High resolution limit [Å] | 1.501 | 1.500 |
Rmerge | 0.060 | 0.210 |
Total number of observations | 358628 * | |
Number of reflections | 95355 * | |
<I/σ(I)> | 7.9 | 3.6 |
Completeness [%] | 97.0 | 96 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.2 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 0.6-0.8 (M) | |
3 | 1 | drop | sodium acetate | 0.05 (M) | |
4 | 1 | reservoir | ammonium sulfate | 1.2-1.4 (M) |