1H9D

Aml1/cbf-beta/dna complex

1H9D の概要

• エントリーDOI: 10.2210/pdb1h9d/pdb
• 関連するPDBエントリー: 1CL3 1CMO 1CO1 1E50
• 分子名称: CORE-BINDING FACTOR ALPHA SUBUNIT1, CORE-BINDING FACTOR CBF-BETA, DNA (5'-(*GP*TP*TP*GP*CP*GP*GP*TP*TP*G)-3'), ... (5 entities in total)
• 機能のキーワード: transcription factor
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: Q01196 Q13951
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 73629.69

構造登録者

Bravo, J.,Warren, A.J. (登録日: 2001-03-07, 公開日: 2001-03-31, 最終更新日: 2023-12-13)

主引用文献

Bravo, J.,Li, Z.,Speck, N.A.,Warren, A.J.
The Leukemia-Associated Aml1 (Runx1)-Cbfbeta Complex Functions as a DNA-Induced Molecular Clamp
Nat.Struct.Biol., 8:371-, 2001

PubMed Abstract: We have determined the structure, at 2.6 A resolution, of the AML1 (Runx1) Runt domain--CBF beta--DNA ternary complex, the most common target for mutations in human leukemia. The structure reveals that the Runt domain DNA binding mechanism is unique within the p53 family of transcription factors. The extended C-terminal 'tail' and 'wing' elements adopt a specific DNA-bound conformation that clamps the phosphate backbone between the major and minor grooves of the distorted B-form DNA recognition site. Furthermore, the extended 'tail' mediates most of the NF-kappa B/Rel-like base-specific contacts in the major groove. The structure clearly explains the molecular basis for the loss of DNA binding function of the Runt domain--CBF beta complex as a consequence of the human disease-associated mutations in leukemogenesis and cleidocranial dysplasia.

PubMed: 11276260
DOI: 10.1038/86264

実験手法

X-RAY DIFFRACTION (2.6 Å)