1H9D
Aml1/cbf-beta/dna complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 115.033, 115.033, 133.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.020 - 2.600 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e50 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.126 * | 0.380 * |
| Total number of observations | 193723 * | |
| Number of reflections | 27926 | |
| <I/σ(I)> | 19.4 | 2.2 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 23 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 300 (mM) | ||
| 2 | 1 | drop | MES | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | HEPES | 50 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 3 (%(w/v)) | |
| 7 | 1 | reservoir | dithiothreitol | 5 (mM) |
