1H34

Crystal structure of lima bean trypsin inhibitor

Summary for 1H34

• Entry DOI: 10.2210/pdb1h34/pdb
• Descriptor: BOWMAN-BIRK TYPE PROTEINASE INHIBITOR (2 entities in total)
• Functional Keywords: inhibitor, bowman-birk-type proteinase inhibitor, serine protease inhibitor
• Biological source: PHASEOLUS LUNATUS (LIMA BEAN)
• Total number of polymer chains: 1
• Total formula weight: 9043.96

Authors

Debreczeni, J.E.,Bunkoczi, G.,Girmann, B.,Sheldrick, G.M. (deposition date: 2002-08-21, release date: 2003-02-06, Last modification date: 2024-11-20)

Primary citation

Debreczeni, J.E.,Bunkoczi, G.,Girmann, B.,Sheldrick, G.M.
In-House Phase Determination of the Lima Bean Trypsin Inhibitor: A Low-Resolution Sulfur-Sad Case
Acta Crystallogr.,Sect.D, 59:393-, 2003

PubMed Abstract: SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.

PubMed: 12554963
DOI: 10.1107/S0907444902020917

Experimental method

X-RAY DIFFRACTION (2.04 Å)