1GXR

WD40 Region of Human Groucho/TLE1

Summary for 1GXR

• Entry DOI: 10.2210/pdb1gxr/pdb
• Descriptor: TRANSDUCIN-LIKE ENHANCER PROTEIN 1, CALCIUM ION (3 entities in total)
• Functional Keywords: transcriptional co-repressor, wd40, transcription repressor, wd repeat, transcription
• Biological source: HOMO SAPIENS (HUMAN)
• Total number of polymer chains: 2
• Total formula weight: 73747.02

Authors

Pearl, L.H.,Roe, S.M.,Pickles, L.M. (deposition date: 2002-04-10, release date: 2002-06-13, Last modification date: 2023-12-13)

Primary citation

Pickles, L.M.,Roe, S.M.,Hemingway, E.J.,Stifani, S.,Pearl, L.H.
Crystal Structure of the C-Terminal Wd40 Repeat Domain of the Human Groucho/Tle1 Transcriptional Corepressor
Structure, 10:751-, 2002

PubMed Abstract: Groucho (Gro)/TLE proteins are transcriptional corepressors that lack inherent DNA binding but interact with DNA-bound transcription factors and histones, and recruit histone deacetylases. Groucho-mediated repression is essential in embryonic development and involved in regulation of Wnt signaling in adult tissue. We have determined the 1.6 A crystal structure of a C-terminal fragment of human Groucho/TLE1, comprising part of the Ser/Pro-rich region and a seven-bladed beta propeller WD40 repeat domain, implicated in protein-protein interactions. The structure confirms the relationship to the yeast Tup1 corepressor, but reveals important structural differences specific to the metazoan system. Analysis of missense mutations in the C. elegans Groucho homolog UNC-37 identifies sites of interaction with repression effectors, and suggests an induced fit binding site for eh1 domains of Engrailed-type transcription factors.

PubMed: 12057191
DOI: 10.1016/S0969-2126(02)00768-2

Experimental method

X-RAY DIFFRACTION (1.65 Å)