1GXR

WD40 Region of Human Groucho/TLE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	62.407, 56.656, 102.034
Unit cell angles	90.00, 102.29, 90.00

Refinement procedure

Resolution	40.450 - 1.650
R-factor	0.23 *
Rwork	0.229
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1erj
RMSD bond length	0.005
RMSD bond angle	26.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.670
High resolution limit [Å]	1.650 *	1.600
Rmerge	0.064	0.273
Number of reflections	83990 *
<I/σ(I)>	5.4	2.6
Completeness [%]	100.0	100
Redundancy	5.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7	14 *	CRYSTALS WERE GROWN BY MICROBATCH METHODS AT 14C. PROTEIN AT 6MG/ML, WAS MIXED WITH AN EQUAL VOLUME O PRECIPITANT (22% PEG8000, 100MM, NACACODYLATE, 100MM CAACETATE)., pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	6 (mg/ml)
2	1	1	PEG8000	22 (%)
3	1	1	sodium cacodylate	100 (mM)
4	1	1	calcium acetate	100 (mM)