1GSZ
Crystal Structure of a Squalene Cyclase in Complex with the Potential Anticholesteremic Drug Ro48-8071
Summary for 1GSZ
Entry DOI | 10.2210/pdb1gsz/pdb |
Related | 1SQC 2SQC 3SQC |
Descriptor | SQUALENE--HOPENE CYCLASE, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE, [4-({6-[ALLYL(METHYL)AMINO]HEXYL}OXY)-2-FLUOROPHENYL](4-BROMOPHENYL)METHANONE, ... (4 entities in total) |
Functional Keywords | isomerase, cholesterol biosynthesis, inhibitor oxidosqualene cyclase, monotopic membrane protein |
Biological source | ALICYCLOBACILLUS ACIDOCALDARIUS |
Total number of polymer chains | 3 |
Total formula weight | 217214.54 |
Authors | Lenhart, A.,Weihofen, W.A.,Pleschke, A.E.W.,Schulz, G.E. (deposition date: 2002-01-09, release date: 2003-01-16, Last modification date: 2024-05-08) |
Primary citation | Lenhart, A.,Weihofen, W.A.,Pleschke, A.E.W.,Schulz, G.E. Crystal Structure of a Squalene Cyclase in Complex with the Potential Anticholesteremic Drug Ro48-8071 Chem.Biol., 9:639-, 2002 Cited by PubMed Abstract: Squalene-hopene cyclase (SHC) catalyzes the conversion of squalene into pentacyclic compounds. It is the prokaryotic counterpart of the eukaryotic oxidosqualene cyclase (OSC) that catalyzes the steroid scaffold formation. Because of clear sequence homology, SHC can serve as a model for OSC, which is an attractive target for anticholesteremic drugs. We have established the crystal structure of SHC complexed with Ro48-8071, a potent inhibitor of OSC and therefore of cholesterol biosynthesis. Ro48-8071 is bound in the active-center cavity of SHC and extends into the channel that connects the cavity with the membrane. The binding site of Ro48-8071 is largely identical with the expected site of squalene; it differs from a previous model based on photoaffinity labeling. The knowledge of the inhibitor binding mode in SHC is likely to help develop more potent inhibitors for OSC. PubMed: 12031670DOI: 10.1016/S1074-5521(02)00138-2 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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