1GQ8

Pectin methylesterase from Carrot

Summary for 1GQ8

Entry DOI	10.2210/pdb1gq8/pdb
Descriptor	PECTINESTERASE, CACODYLATE ION (3 entities in total)
Functional Keywords	hydrolase, carboxylic ester hydrolase
Biological source	DAUCUS CAROTA (CARROT)
Total number of polymer chains	1
Total formula weight	34500.22
Authors	Johansson, K.,El-Ahmad, M.,Friemann, R.,Jornvall, H.,Markovic, O.,Eklund, H. (deposition date: 2001-11-20, release date: 2002-04-18, Last modification date: 2024-10-23)
Primary citation	Johansson, K.,El-Ahmad, M.,Friemann, R.,Jornvall, H.,Markovic, O.,Eklund, H. Crystal Structure of Plant Pectin Methylesterase FEBS Lett., 514:243-, 2002 Cited by PubMed Abstract: Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The beta-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state. PubMed: 11943159 DOI: 10.1016/S0014-5793(02)02372-4 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.75 Å)

Structure validation