1GQ8
Pectin methylesterase from Carrot
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-12 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.690, 89.410, 49.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.260 - 1.750 |
R-factor | 0.182 * |
Rwork | 0.181 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qjv |
RMSD bond length | 0.008 * |
RMSD bond angle | 26.200 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.073 | 0.291 |
Number of reflections | 35380 * | |
<I/σ(I)> | 9.6 | 2.6 |
Completeness [%] | 99.6 | 98.6 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 14 * | 0.2M NA ACETATE, 0.1M NA CACODYLATE PH 6.5, 30% PEG8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 0.2 (M) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
3 | 1 | reservoir | PEG8000 | 30 (%) | |
4 | 1 | drop | protein | 10 (mg/ml) |