1GP7

Acidic Phospholipase A2 from venom of Ophiophagus Hannah

Summary for 1GP7

• Entry DOI: 10.2210/pdb1gp7/pdb
• Descriptor: PHOSPHOLIPASE A2, CALCIUM ION (3 entities in total)
• Functional Keywords: phospholipase a2, snake venom, king cobra, cardiotoxic activity, myotoxic activity, pancreatic loop, hydrolase, lipid degradation, calcium
• Biological source: OPHIOPHAGUS HANNAH (KING COBRA)
• Cellular location: Secreted: P80966
• Total number of polymer chains: 3
• Total formula weight: 49490.34

Authors

Zhang, H.,Lin, Z. (deposition date: 2001-10-30, release date: 2002-10-31, Last modification date: 2024-11-20)

Primary citation

Zhang, H.,Xu, S.,Wang, Q.,Song, S.,Shu, Y.,Lin, Z.
Structure of a Cardiotoxic Phospholipase A(2) from Ophiophagus Hannah with the "Pancreatic Loop"
J.Struct.Biol., 138:207-, 2002

PubMed Abstract: The crystal structure of an acidic phospholipase A(2) from Ophiophagus hannah (king cobra) has been determined by molecular replacement at 2.6-A resolution to a crystallographic R factor of 20.5% (R(free)=23.3%) with reasonable stereochemistry. The venom enzyme contains an unusual "pancreatic loop." The conformation of the loop is well defined and different from those in pancreas PLA(2), showing its structural variability. This analysis provides the first structure of a PLA(2)-type cardiotoxin. The sites related to the cardiotoxic and myotoxic activities are explored and the oligomer observed in the crystalline state is described.

PubMed: 12217659
DOI: 10.1016/S1047-8477(02)00022-9

Experimental method

X-RAY DIFFRACTION (2.6 Å)