1GP7
Acidic Phospholipase A2 from venom of Ophiophagus Hannah
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.920, 62.940, 57.160 |
| Unit cell angles | 90.00, 100.93, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1poa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.087 | 0.205 |
| Total number of observations | 32758 * | |
| Number of reflections | 11965 * | |
| <I/σ(I)> | 15 | 5 |
| Completeness [%] | 93.8 | 75 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 291 * | Wang, Z., (2001) Acta Crystallogr.,Sect.D, D57, 709. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 5 (%) | |
| 3 | 1 | drop | ethylene glycol | 4 (%) | |
| 4 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 5 | 1 | reservoir | PEG4000 | 10 (%) | |
| 6 | 1 | reservoir | ethylene glycol | 8 (%) |
