Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

1G83

CRYSTAL STRUCTURE OF FYN SH3-SH2

Summary for 1G83
Entry DOI10.2210/pdb1g83/pdb
DescriptorPROTO-ONCOGENE TYROSINE-PROTEIN KINASE FYN (2 entities in total)
Functional Keywordsbeta barrel, antiparallel beta sheet, alpha helix, 3-10 helix, transferase
Biological sourceHomo sapiens (human)
Cellular locationCell membrane: P06241
Total number of polymer chains2
Total formula weight37821.86
Authors
Arold, S.T.,Ulmer, T.S.,Mulhern, T.D.,Werner, J.M.,Ladbury, J.E.,Campbell, I.D.,Noble, M.E.M. (deposition date: 2000-11-16, release date: 2001-05-30, Last modification date: 2024-02-07)
Primary citationArold, S.T.,Ulmer, T.S.,Mulhern, T.D.,Werner, J.M.,Ladbury, J.E.,Campbell, I.D.,Noble, M.E.
The role of the Src homology 3-Src homology 2 interface in the regulation of Src kinases.
J.Biol.Chem., 276:17199-17205, 2001
Cited by
PubMed Abstract: The regulatory fragment of Src kinases, comprising Src homology (SH) 3 and SH2 domains, is responsible for controlled repression of kinase activity. We have used a multidisciplinary approach involving crystallography, NMR, and isothermal titration calorimetry to study the regulatory fragment of Fyn (FynSH32) and its interaction with a physiological activator: a fragment of focal adhesion kinase that contains both phosphotyrosine and polyproline motifs. Although flexible, the preferred disposition of SH3 and SH2 domains in FynSH32 resembles the inactive forms of Hck and Src, differing significantly from LckSH32. This difference, which results from variation in the SH3-SH2 linker sequences, will affect the potential of the regulatory fragments to repress kinase activity. This surprising result implies that the mechanism of repression of Src family members may vary, explaining functional distinctions between Fyn and Lck. The interaction between FynSH32 and focal adhesion kinase is restricted to the canonical SH3 and SH2 binding sites and does not affect the dynamic independence of the two domains. Consequently, the interaction shows no enhancement by an avidity effect. Such an interaction may have evolved to gain specificity through an extended recognition site while maintaining rapid dissociation after signaling.
PubMed: 11278857
DOI: 10.1074/jbc.M011185200
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.6 Å)
Structure validation

238582

PDB entries from 2025-07-09

PDB statisticsPDBj update infoContact PDBjnumon