1G83
CRYSTAL STRUCTURE OF FYN SH3-SH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 1999-04-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9402 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.970, 89.960, 60.270 |
| Unit cell angles | 90.00, 101.44, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.600 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.660 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 * | 0.430 |
| Total number of observations | 69967 * | |
| Number of reflections | 10431 * | |
| <I/σ(I)> | 6.3 | 1.5 |
| Completeness [%] | 82.0 | 83.4 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 295 | PEG 8000, sodium tartrate, TRIS , pH 6.0, VAPOR DIFFUSION, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | protein | 16 (mg/ml) | |
| 4 | 1 | reservoir | sodium tartrate | 0.25 (M) | |
| 5 | 1 | reservoir | PEG8000 | 12 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
