1G2W

E177S MUTANT OF THE PYRIDOXAL-5'-PHOSPHATE ENZYME D-AMINO ACID AMINOTRANSFERASE

Summary for 1G2W

Related1DAA 2DAA 3DAA 4DAA 5DAA 2DAB 1A0G
DescriptorD-ALANINE AMINOTRANSFERASE, ACETATE ION, PYRIDOXAL-5'-PHOSPHATE, ... (4 entities in total)
Functional Keywordsmutant, pyridoxal-5'-phosphate, water molecule, internal aldimine, aminotransferase, bacterial cell wall biosynthesis, transferase
Biological sourceGeobacillus stearothermophilus
Total number of polymer chains2
Total molecular weight65152.12
Authors
Lepore, B.W.,Ringe, D. (deposition date: 2000-10-21, release date: 2000-11-15, Last modification date: 2011-07-13)
Primary citation
van Ophem, P.W.,Lepore, B.W.,Kishimoto, K.,Ringe, D.,Manning, J.M.
Studies on an Active Site Residue, E177, That Affects Binding of the Coenzyme in D-Amino Acid Transaminase, and Mechanistic Studies on a Suicide Substrate
Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins, 10th Annual International Symposium on Vitamin B6, :339-346, 2000
Experimental method
X-RAY DIFFRACTION (2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.21940.2%1.4%1.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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