1G2W
E177S MUTANT OF THE PYRIDOXAL-5'-PHOSPHATE ENZYME D-AMINO ACID AMINOTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-29 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.187, 90.522, 89.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.000 * - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.590 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.140 |
| High resolution limit [Å] | 1.800 | 1.800 * |
| Rmerge | 0.123 | 0.244 * |
| Total number of observations | 303396 * | |
| Number of reflections | 96070 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 85.4 * | 97.5 * |
| Redundancy | 3.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 * | 277 | resevoir: Tris-HCl, pH 8.5, 22-26% PEG 4K, 0.2-0.4M NaOAc, 1mM alpha-ketoglutarate, enzyme buffer: 50mM KPO4, pH 7.2, 200mM KCl, 1mM DTT, 10uM PLP , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 30 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | 0.13 (M) | ||
| 4 | 1 | drop | EDTA | 0.2 (mM) | |
| 5 | 1 | drop | PLP | 50 (mM) | |
| 6 | 1 | drop | beta-mercaptoethanol | 0.01 (%) | |
| 7 | 1 | reservoir | PEG3350 | 26 (%) | |
| 8 | 1 | reservoir | sodium acetate | 0.3 (M) | |
| 9 | 1 | reservoir | Tris-HCl | 0.1 (M) |
