1G1I
CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4
Summary for 1G1I
| Entry DOI | 10.2210/pdb1g1i/pdb |
| Related | 1G1J |
| Descriptor | NON-STRUCTURAL GLYCOPROTEIN NSP4, CALCIUM ION (3 entities in total) |
| Functional Keywords | ns28, parallel coiled-coil, homo-tetramer, metal binding site, metal binding protein |
| Total number of polymer chains | 2 |
| Total formula weight | 10848.59 |
| Authors | Bowman, G.D.,Nodelman, I.M.,Schutt, C.E. (deposition date: 2000-10-11, release date: 2001-01-10, Last modification date: 2024-10-30) |
| Primary citation | Bowman, G.D.,Nodelman, I.M.,Levy, O.,Lin, S.L.,Tian, P.,Zamb, T.J.,Udem, S.A.,Venkataraghavan, B.,Schutt, C.E. Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site. J.Mol.Biol., 304:861-871, 2000 Cited by PubMed Abstract: During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin. PubMed: 11124032DOI: 10.1006/jmbi.2000.4250 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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