1G1I
CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-27 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9785,0.9788,0.95 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 35.470, 35.399, 60.937 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.070 - 2.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.045 | 0.235 |
| Total number of observations | 109286 * | |
| Number of reflections | 10000 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | PEG400, magnesium sulfate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | PEG400 | 20-28 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) |
