1FZV

THE CRYSTAL STRUCTURE OF HUMAN PLACENTA GROWTH FACTOR-1 (PLGF-1), AN ANGIOGENIC PROTEIN AT 2.0A RESOLUTION

Summary for 1FZV

• Entry DOI: 10.2210/pdb1fzv/pdb
• Related: 1FLT 2VPF
• Descriptor: PLACENTA GROWTH FACTOR, (4S)-2-METHYL-2,4-PENTANEDIOL (3 entities in total)
• Functional Keywords: cysteine-knot family, growth factor, angiogenesis, hormone-growth factor complex, hormone/growth factor
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P49763
• Total number of polymer chains: 2
• Total formula weight: 29980.48

Authors

Iyer, S.,Leonidas, D.D.,Swaminathan, G.J.,Maglione, D.,Battisti, M.,Tucci, M.,Persico, M.G.,Acharya, K.R. (deposition date: 2000-10-04, release date: 2001-05-09, Last modification date: 2024-10-16)

Primary citation

Iyer, S.,Leonidas, D.D.,Swaminathan, G.J.,Maglione, D.,Battisti, M.,Tucci, M.,Persico, M.G.,Acharya, K.R.
The crystal structure of human placenta growth factor-1 (PlGF-1), an angiogenic protein, at 2.0 A resolution.
J.Biol.Chem., 276:12153-12161, 2001

PubMed Abstract: The angiogenic molecule placenta growth factor (PlGF) is a member of the cysteine-knot family of growth factors. In this study, a mature isoform of the human PlGF protein, PlGF-1, was crystallized as a homodimer in the crystallographic asymmetric unit, and its crystal structure was elucidated at 2.0 A resolution. The overall structure of PlGF-1 is similar to that of vascular endothelial growth factor (VEGF) with which it shares 42% amino acid sequence identity. Based on structural and biochemical data, we have mapped several important residues on the PlGF-1 molecule that are involved in recognition of the fms-like tyrosine kinase receptor (Flt-1, also known as VEGFR-1). We propose a model for the association of PlGF-1 and Flt-1 domain 2 with precise shape complementarity, consider the relevance of this assembly for PlGF-1 signal transduction, and provide a structural basis for altered specificity of this molecule.

PubMed: 11069911
DOI: 10.1074/jbc.M008055200

Experimental method

X-RAY DIFFRACTION (2 Å)