1FZV
THE CRYSTAL STRUCTURE OF HUMAN PLACENTA GROWTH FACTOR-1 (PLGF-1), AN ANGIOGENIC PROTEIN AT 2.0A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-27 |
Detector | MARRESEARCH |
Spacegroup name | P 43 |
Unit cell lengths | 62.600, 62.600, 84.107 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.170 - 2.000 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.24700 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.364 |
Total number of observations | 161044 * | |
Number of reflections | 20658 | |
<I/σ(I)> | 19.67 | |
Completeness [%] | 94.1 | 98.8 * |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | MES, MPD and Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 16K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | MES | 0.05 (M) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | MPD | 7.5 (%(v/v)) | |
5 | 1 | reservoir | MES | 0.1 (M) | |
6 | 1 | reservoir | 20 (mM) | ||
7 | 1 | reservoir | MPD | 15 (%(v/v)) |