1FK8

THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX WITH NAD OF 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE FROM COMAMONAS TESTOSTERONI, A MEMBER OF THE SHORT CHAIN DEHYDROGENASE/REDUCTASE FAMILY

1FK8 の概要

• エントリーDOI: 10.2210/pdb1fk8/pdb
• 関連するPDBエントリー: 1FJH
• 分子名称: 3ALPHA-HYDROXYSTEROID DEHYDROGENASE/CARBONYL REDUCTASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: short chain dehydrogenase, sdr, carbonyl reductase, steroid, hydroxysteroid, xenobiotic, metyrapone, oligomerisation, comamonas testosteroni, nad, nicotinamide adenine dinucleotide, substrate binding loop, oxidoreductase
• 由来する生物種: Comamonas testosteroni
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54167.71

構造登録者

Grimm, C.,Ficner, R.,Maser, E.,Klebe, G.,Reuter, K. (登録日: 2000-08-09, 公開日: 2001-01-17, 最終更新日: 2024-02-07)

主引用文献

Grimm, C.,Maser, E.,Mobus, E.,Klebe, G.,Reuter, K.,Ficner, R.
The crystal structure of 3alpha -hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerization pattern within the short chain dehydrogenase/reductase family.
J.Biol.Chem., 275:41333-41339, 2000

PubMed Abstract: The crystal structure of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni (3alpha-HSDH) as well as the structure of its binary complex with NAD(+) have been solved at 1.68-A and 1.95-A resolution, respectively. The enzyme is a member of the short chain dehydrogenase/reductase (SDR) family. Accordingly, the active center and the conformation of the bound nucleotide cofactor closely resemble those of other SDRs. The crystal structure reveals one homodimer per asymmetric unit representing the physiologically active unity. Dimerization takes place via an interface essentially built-up by helix alphaG and strand betaG of each subunit. So far this type of intermolecular contact has exclusively been observed in homotetrameric SDRs but never in the structure of a homodimeric SDR. The formation of a tetramer is blocked in 3alpha-HSDH by the presence of a predominantly alpha-helical subdomain which is missing in all other SDRs of known structure.

PubMed: 11007791
DOI: 10.1074/jbc.M007559200

実験手法

X-RAY DIFFRACTION (1.95 Å)