1FJL

HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE

Summary for 1FJL

• Entry DOI: 10.2210/pdb1fjl/pdb
• Descriptor: PAIRED PROTEIN, DNA (5'-D(*AP*AP*TP*AP*AP*TP*CP*TP*GP*AP*TP*TP*AP*C)-3'), DNA (5'-D(*TP*GP*TP*AP*AP*TP*CP*AP*GP*AP*TP*TP*AP*T)-3'), ... (5 entities in total)
• Functional Keywords: dna-binding protein, dna, paired box, transcription regulation, transcription-dna complex, transcription/dna
• Biological source: Drosophila melanogaster (fruit fly)
• Cellular location: Nucleus: P06601
• Total number of polymer chains: 6
• Total formula weight: 41976.79

Authors

Wilson, D.S.,Guenther, B.,Desplan, C.,Kuriyan, J. (deposition date: 1995-12-17, release date: 1996-06-20, Last modification date: 2024-02-07)

Primary citation

Wilson, D.S.,Guenther, B.,Desplan, C.,Kuriyan, J.
High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA.
Cell(Cambridge,Mass.), 82:709-719, 1995

PubMed Abstract: The crystal structure of the paired homeodomain bound to DNA as a cooperative dimer has been determined to 2.0 A resolution. Direct contacts between each homeodomain and the DNA are similar to those described previously. In addition, an extensive network of water molecules mediates contacts between the recognition helix and the DNA major groove. Several symmetrical contacts between the two homeodomains underlie the cooperative interaction, and deformations in the DNA structure are necessary for the establishment of these contacts. Comparison with structures of homeodomains bound monomerically to DNA suggests that the binding of a single paired homeodomain can introduce these DNA distortions, thus preparing a template for the cooperative interaction with a second homeodomain. This study shows how the paired (Pax) class homeodomains have achieved cooperativity in DNA binding without the assistance of other domains, thereby enabling the recognition of target sequences that are long enough to ensure specificity.

PubMed: 7671301
DOI: 10.1016/0092-8674(95)90468-9

Experimental method

X-RAY DIFFRACTION (2 Å)