1FHZ

PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION

Summary for 1FHZ

• Entry DOI: 10.2210/pdb1fhz/pdb
• Descriptor: DNA (5'-D(*CP*CP*GP*GP*TP*AP*CP*CP*GP*G)-3'), 4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN (3 entities in total)
• Functional Keywords: psoralen, cross-linked dna, holliday junction, dna-drug complex, dna
• Total number of polymer chains: 2
• Total formula weight: 6350.25

Authors

Eichman, B.F.,Mooers, B.H.M.,Alberti, M.,Hearst, J.E.,Ho, P.S. (deposition date: 2000-08-02, release date: 2001-04-21, Last modification date: 2024-02-07)

Primary citation

Eichman, B.F.,Mooers, B.H.,Alberti, M.,Hearst, J.E.,Ho, P.S.
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions.
J.Mol.Biol., 308:15-26, 2001

PubMed Abstract: The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

PubMed: 11302703
DOI: 10.1006/jmbi.2001.4567

Experimental method

X-RAY DIFFRACTION (2.2 Å)