1FHZ
PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1999-10-08 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.116, 23.651, 36.182 |
| Unit cell angles | 90.00, 112.64, 90.00 |
Refinement procedure
| Resolution | 33.400 * - 2.200 |
| R-factor | 0.236 |
| Rwork | 0.228 |
| R-free | 0.28400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.229 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.400 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.100 |
| Total number of observations | 9596 * | |
| Number of reflections | 2900 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 96.7 | 98.3 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 277 | sodium cacodylate, MgCl2, MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 30 (mM) | ||
| 2 | 1 | drop | DNA | 0.5 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | MPD | 10 (%) | |
| 5 | 1 | reservoir | 20 (%(v/v)) |
