1FHW

Structure of the pleckstrin homology domain from GRP1 in complex with inositol(1,3,4,5,6)pentakisphosphate

1FHW の概要

• エントリーDOI: 10.2210/pdb1fhw/pdb
• 関連するPDBエントリー: 1FHX
• 分子名称: GUANINE NUCLEOTIDE EXCHANGE FACTOR AND INTEGRIN BINDING PROTEIN HOMOLOG GRP1, INOSITOL-(1,3,4,5,6)-PENTAKISPHOSPHATE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: pleckstrin, 3-phosphoinositides, inositol tetrakisphosphate signal transduction protein, guanine nucleotide exchange factor, signaling protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31694.99

構造登録者

Ferguson, K.M.,Kavran, J.M.,Sankaran, V.G.,Fournier, E.,Isakoff, S.J.,Skolnik, E.Y.,Lemmon, M.A. (登録日: 2000-08-02, 公開日: 2000-08-23, 最終更新日: 2024-11-13)

主引用文献

Ferguson, K.M.,Kavran, J.M.,Sankaran, V.G.,Fournier, E.,Isakoff, S.J.,Skolnik, E.Y.,Lemmon, M.A.
Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains
Mol.Cell, 6:373-384, 2000

PubMed Abstract: Pleckstrin homology (PH) domains are protein modules of around 120 amino acids found in many proteins involved in cellular signaling. Certain PH domains drive signal-dependent membrane recruitment of their host proteins by binding strongly and specifically to lipid second messengers produced by agonist-stimulated phosphoinositide 3-kinases (PI 3-Ks). We describe X-ray crystal structures of two different PH domains bound to Ins(1,3,4,5)P4, the head group of the major PI 3-K product PtdIns(3,4,5)P3. One of these PH domains (from Grp1) is PtdIns(3,4,5)P3 specific, while the other (from DAPP1/PHISH) binds strongly to both PtdIns(3,4,5)P3 and its 5'-dephosphorylation product, PtdIns(3,4)P2. Comparison of the two structures provides an explanation for the distinct phosphoinositide specificities of the two PH domains and allows us to predict the 3-phosphoinositide selectivity of uncharacterized PH domains.

PubMed: 10983984
DOI: 10.1016/S1097-2765(00)00037-X

実験手法

X-RAY DIFFRACTION (1.9 Å)