1FHW
Structure of the pleckstrin homology domain from GRP1 in complex with inositol(1,3,4,5,6)pentakisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-10 |
Detector | BRANDEIS - B4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.970, 72.220, 46.900 |
Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
Resolution | 500.000 * - 1.900 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27000 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.080 |
Total number of observations | 53932 * | |
Number of reflections | 18374 | |
<I/σ(I)> | 20 | 10.7 |
Completeness [%] | 95.2 | 80.5 |
Redundancy | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 21 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 2-10 (%) | |
2 | 1 | reservoir | sodium acetate | 50 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
4 | 1 | drop | protein | 15 (mg/ml) | |
5 | 1 | drop | MES | 10 (mM) | |
6 | 1 | drop | 100 (mM) |