1FBV

STRUCTURE OF A CBL-UBCH7 COMPLEX: RING DOMAIN FUNCTION IN UBIQUITIN-PROTEIN LIGASES

Summary for 1FBV

• Entry DOI: 10.2210/pdb1fbv/pdb
• Descriptor: SIGNAL TRANSDUCTION PROTEIN CBL, ZAP-70 PEPTIDE, UBIQUITIN-CONJUGATING ENZYME E12-18 KDA UBCH7, ... (5 entities in total)
• Functional Keywords: cbl, ubch7, zap-70, e2, ubiquitin, e3, phosphorylation, tyrosine kinase, ubiquitination, protein degradation, ligase
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: P22681; Nucleus : P68036
• Total number of polymer chains: 3
• Total formula weight: 64330.24

Authors

Zheng, N.,Wang, P.,Jeffrey, P.D.,Pavletich, N.P. (deposition date: 2000-07-17, release date: 2000-08-30, Last modification date: 2024-10-30)

Primary citation

Zheng, N.,Wang, P.,Jeffrey, P.D.,Pavletich, N.P.
Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases.
Cell(Cambridge,Mass.), 102:533-539, 2000

PubMed Abstract: Ubiquitin-protein ligases (E3s) regulate diverse cellular processes by mediating protein ubiquitination. The c-Cbl proto-oncogene is a RING family E3 that recognizes activated receptor tyrosine kinases, promotes their ubiquitination by a ubiquitin-conjugating enzyme (E2) and terminates signaling. The crystal structure of c-Cbl bound to a cognate E2 and a kinase peptide shows how the RING domain recruits the E2. A comparison with a HECT family E3-E2 complex indicates that a common E2 motif is recognized by the two E3 families. The structure reveals a rigid coupling between the peptide binding and the E2 binding domains and a conserved surface channel leading from the peptide to the E2 active site, suggesting that RING E3s may function as scaffolds that position the substrate and the E2 optimally for ubiquitin transfer.

PubMed: 10966114
DOI: 10.1016/S0092-8674(00)00057-X

Experimental method

X-RAY DIFFRACTION (2.9 Å)