1FBV

STRUCTURE OF A CBL-UBCH7 COMPLEX: RING DOMAIN FUNCTION IN UBIQUITIN-PROTEIN LIGASES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 41 3 2
Unit cell lengths	219.000, 219.000, 219.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.900
R-factor	0.227 *
Rwork	0.227
R-free	0.26200
RMSD bond length	0.011 *
RMSD bond angle	1.920 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall
High resolution limit [Å]	2.900 *
Rmerge	0.062 *
Total number of observations	156681 *
Number of reflections	38512 *
Completeness [%]	96.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	4 *	c-Cbl:UbcH7:ZAP-70=1:1:3 at a molar ratio in a solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	c-Cbl	27 (mg/ml)
2	1	drop	UbcH7	32 (mg/ml)
3	1	drop	ZAP-70	40 (mg/ml)
4	1	drop	2-(N-Morpholino)ethanesulfonic acid	20 (mM)
5	1	drop		150 (mM)
6	1	drop	dithiothreitol	5 (mM)
7	1	reservoir	sodium citrate	100 (mM)
8	1	reservoir	ammonium sulfate	1.9-2.1 (M)