1F4X

CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN

Summary for 1F4X

• Entry DOI: 10.2210/pdb1f4x/pdb
• Related: 1F4W 1F4Y
• Descriptor: ANTIBODY S-20-4, FAB FRAGMENT, LIGHT CHAIN, ANTIBODY S-20-4, FAB FRAGMENT, HEAVY CHAIN, methyl 4,6-dideoxy-4-{[(2R)-2,4-dihydroxybutanoyl]amino}-2-O-methyl-alpha-D-mannopyranoside, ... (4 entities in total)
• Functional Keywords: anti-carbohydrate antibody, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 46080.33

Authors

Alzari, P.M.,Souchon, H. (deposition date: 2000-06-10, release date: 2000-08-02, Last modification date: 2024-10-30)

Primary citation

Villeneuve, S.,Souchon, H.,Riottot, M.M.,Mazie, J.C.,Lei, P.,Glaudemans, C.P.,Kovac, P.,Fournier, J.M.,Alzari, P.M.
Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.
Proc.Natl.Acad.Sci.USA, 97:8433-8438, 2000

PubMed Abstract: The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.

PubMed: 10880560
DOI: 10.1073/pnas.060022997

Experimental method

X-RAY DIFFRACTION (2.3 Å)