1F4W
CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN
Summary for 1F4W
Entry DOI | 10.2210/pdb1f4w/pdb |
Related | 1F4X 1F4Y |
Descriptor | ANTIBODY S-20-4, FAB FRAGMENT, LIGHT CHAIN, ANTIBODY S-20-4, FAB FRAGMENT, HEAVY CHAIN (3 entities in total) |
Functional Keywords | anti-carbohydrate antibody, immune system |
Biological source | Mus musculus (house mouse) More |
Total number of polymer chains | 2 |
Total formula weight | 45787.02 |
Authors | Alzari, P.M.,Souchon, H. (deposition date: 2000-06-10, release date: 2000-08-02, Last modification date: 2024-10-30) |
Primary citation | Villeneuve, S.,Souchon, H.,Riottot, M.M.,Mazie, J.C.,Lei, P.,Glaudemans, C.P.,Kovac, P.,Fournier, J.M.,Alzari, P.M. Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity. Proc.Natl.Acad.Sci.USA, 97:8433-8438, 2000 Cited by PubMed Abstract: The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine. PubMed: 10880560DOI: 10.1073/pnas.060022997 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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