1EQP

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS

Summary for 1EQP

• Entry DOI: 10.2210/pdb1eqp/pdb
• Related: 1CZ1 1EQC
• Descriptor: EXO-B-(1,3)-GLUCANASE (2 entities in total)
• Functional Keywords: candida albicans, exoglucanase, alternative codon usage, hydrolase
• Biological source: Candida albicans
• Cellular location: Secreted, cell wall : P29717
• Total number of polymer chains: 1
• Total formula weight: 45243.80

Authors

Cutfield, J.F.,Sullivan, P.A.,Cutfield, S.M. (deposition date: 2000-04-05, release date: 2001-02-28, Last modification date: 2024-10-30)

Primary citation

Cutfield, J.F.,Sullivan, P.A.,Cutfield, S.M.
Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase.
Protein Eng., 13:735-738, 2000

PubMed Abstract: In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production.

PubMed: 11112513
DOI: 10.1093/protein/13.10.735

Experimental method

X-RAY DIFFRACTION (1.9 Å)