1E6E

ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS

1E6E の概要

• エントリーDOI: 10.2210/pdb1e6e/pdb
• 関連するPDBエントリー: 1AYF 1CJC 1CJE 1E1K 1E1L 1E1M 1E1N
• 分子名称: NADPH\:ADRENODOXIN OXIDOREDUCTASE, ADRENODOXIN, FLAVIN-ADENINE DINUCLEOTIDE, ... (6 entities in total)
• 機能のキーワード: flavoenzyme, electron transferase, oxidoreductase, [2fe-2s]ferredoxin, adrenodoxin, electron transport, complex
• 由来する生物種: BOS TAURUS (BOVINE); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 131187.34

構造登録者

Mueller, J.J.,Lapko, A.,Bourenkov, G.,Ruckpaul, K.,Heinemann, U. (登録日: 2000-08-15, 公開日: 2001-08-09, 最終更新日: 2023-12-13)

主引用文献

Mueller, J.J.,Lapko, A.,Bourenkov, G.,Ruckpaul, K.,Heinemann, U.
Adrenodoxin Reductase-Adrenodoxin Complex Structure Suggests Electron Transfer Path in Steroid Biosynthesis.
J.Biol.Chem., 276:2786-, 2001

PubMed Abstract: The steroid hydroxylating system of adrenal cortex mitochondria consists of the membrane-attached NADPH-dependent adrenodoxin reductase (AR), the soluble one-electron transport protein adrenodoxin (Adx), and a membrane-integrated cytochrome P450 of the CYP11 family. In the 2.3-A resolution crystal structure of the Adx.AR complex, 580 A(2) of partly polar surface are buried. Main interaction sites are centered around Asp(79), Asp(76), Asp(72), and Asp(39) of Adx and around Arg(211), Arg(240), Arg(244), and Lys(27) of AR, respectively. In particular, the region around Asp(39) defines a new protein interaction site for Adx, similar to those found in plant and bacterial ferredoxins. Additional contacts involve the electron transfer region between the redox centers of AR and Adx and C-terminal residues of Adx. The Adx residues Asp(113) to Arg(115) adopt 3(10)-helical conformation and engage in loose intermolecular contacts within a deep cleft of AR. Complex formation is accompanied by a slight domain rearrangement in AR. The [2Fe-2S] cluster of Adx and the isoalloxazine rings of FAD of AR are 10 A apart suggesting a possible electron transfer route between these redox centers. The AR.Adx complex represents the first structure of a biologically relevant complex between a ferredoxin and its reductase.

PubMed: 11053423
DOI: 10.1074/JBC.M008501200

実験手法

X-RAY DIFFRACTION (2.3 Å)