1E1N

Structure of adrenodoxin reductase at 2.4 Angstrom in crystal form A'

Summary for 1E1N

• Entry DOI: 10.2210/pdb1e1n/pdb
• Related: 1CJC 1E1K 1E1L 1E1M
• Descriptor: ADRENODOXIN REDUCTASE, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• Functional Keywords: oxidoreductase, flavoenzyme, electron transferase
• Biological source: BOS TAURUS
• Cellular location: Mitochondrion matrix: P08165
• Total number of polymer chains: 1
• Total formula weight: 51145.96

Authors

Ziegler, G.A.,Schulz, G.E. (deposition date: 2000-05-09, release date: 2000-09-24, Last modification date: 2024-05-08)

Primary citation

Ziegler, G.A.,Schulz, G.E.
Crystal Structures of Adrenodoxin Reductase in Complex with Nadp+ and Nadph Suggesting a Mechanism for the Electron Transfer of an Enzyme Family
Biochemistry, 39:10986-, 2000

PubMed Abstract: Adrenodoxin reductase is a flavoenzyme that shuffles electrons for the biosynthesis of steroids. Its chain topology belongs to the glutathione reductase family of disulfide oxidoreductases, all of which bind FAD at equivalent positions. The three reported structures of adrenodoxin reductase were ligated with reduced and oxidized NADP and have now confirmed this equivalence also for the NADP-binding site. Remarkably, the conformations and relative positions of the prosthetic group FAD and the cofactor NADP have been conserved during protein evolution despite very substantial changes in the polypeptide. The ligated enzymes showed small changes in the domain positions. When compared with the structure of the NADP-free enzyme, these positions correspond to several states of the domain motion during NADP binding. On the basis of the observed structures, we suggest an enzymatic mechanism for the subdivision of the received two-electron package into the two single electrons transferred to the carrier protein adrenodoxin. The data banks contain 10 sequences that are closely related to bovine adrenodoxin reductase. Most of them code for gene products with unknown functions. Within this family, the crucial residues of adrenodoxin reductase are strictly conserved. Moreover, the putative docking site of the carrier is rather well conserved. Five of the family members were assigned names related to ferredoxin:NADP(+) reductase, presumably because adrenodoxin reductase was considered a member of this functionally similar family. Since this is not the case, the data bank entries should be corrected.

PubMed: 10998235
DOI: 10.1021/BI000079K

Experimental method

X-RAY DIFFRACTION (2.4 Å)