1E1N

Structure of adrenodoxin reductase at 2.4 Angstrom in crystal form A'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Collection date	1998-01-15
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	57.800, 62.000, 83.000
Unit cell angles	90.00, 107.10, 90.00

Refinement procedure

Resolution	29.000 - 2.400
Rwork	0.201
R-free	0.26500
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	0.027
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	2.480
High resolution limit [Å]	2.350	2.350
Rmerge	0.057	0.170
Number of reflections	17479
<I/σ(I)>	11.4	5
Completeness [%]	74.4	36.8
Redundancy	2.4	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	20 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	glycerol	5 (%)
3	1	drop	PEG8000	8 (%(w/v))
4	1	drop	sodium cacodylate	50 (mM)	Buffer C
5	1	drop	calcium acetate	100 (mM)	BufferC
6	1	reservoir	Buffer C
7	1	reservoir	PEG8000	12 (%(w/v))