1E6E

ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 65 2 2
Unit cell lengths	92.210, 92.210, 607.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 2.300
R-factor	0.223 *
Rwork	0.222
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1AYF AND 1CJC
RMSD bond length	0.006 *
RMSD bond angle	1.220 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.069 *	0.120 *
Total number of observations	201839 *
Number of reflections	55229
<I/σ(I)>	18.5	11
Completeness [%]	79.1	56
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	4 *	Lapko, A., (1997) Proteins: Struct.,Funct., Genet., 28, 289. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	complex	1 (mM)
2	1	drop	Tris-HCl	100 (mM)	pH7.4
3	1	drop	ammonium sulfate	1.9 (M)
4	1	reservoir	ammonium sulfate	1.9 (M)