1E6E
ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-15 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.210, 92.210, 607.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.970 - 2.300 |
R-factor | 0.223 * |
Rwork | 0.222 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1AYF AND 1CJC |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.220 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 * | 0.120 * |
Total number of observations | 201839 * | |
Number of reflections | 55229 | |
<I/σ(I)> | 18.5 | 11 |
Completeness [%] | 79.1 | 56 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 4 * | Lapko, A., (1997) Proteins: Struct.,Funct., Genet., 28, 289. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | complex | 1 (mM) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | pH7.4 |
3 | 1 | drop | ammonium sulfate | 1.9 (M) | |
4 | 1 | reservoir | ammonium sulfate | 1.9 (M) |