1E6E
ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.210, 92.210, 607.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 2.300 |
| R-factor | 0.223 * |
| Rwork | 0.222 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1AYF AND 1CJC |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 * | 0.120 * |
| Total number of observations | 201839 * | |
| Number of reflections | 55229 | |
| <I/σ(I)> | 18.5 | 11 |
| Completeness [%] | 79.1 | 56 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 4 * | Lapko, A., (1997) Proteins: Struct.,Funct., Genet., 28, 289. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | complex | 1 (mM) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | pH7.4 |
| 3 | 1 | drop | ammonium sulfate | 1.9 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.9 (M) |
